Research output
Publications
Selected papers and the complete publication record of the group — from capillary electrophoresis and inherited metabolic disorders to clinical lipidomics and multi-omics.
Featured publications
Selected papers representing our main research directions
A curated selection highlighting our work in lipidomics, metabolomics, clinical mass spectrometry and translational laboratory medicine. For the most up-to-date record, see our public profiles above.
Data analysis
Best practices and tools in R and Python for statistical processing and visualization of lipidomics and metabolomics data
A practical high-impact methodological paper providing open tools and recommendations for processing, statistical analysis and visualization of lipidomics and metabolomics data.
DOI Clinical lipidomicsLipidomic profiling of human serum enables detection of pancreatic cancer
A landmark serum lipidomics study demonstrating the potential of lipid profiles for cancer detection and translational biomarker discovery.
DOI Cardiovascular lipidomicsCeramide-based risk score: a novel laboratory tool for cardiovascular risk stratification in hyperuricemia and gout
Shows how ceramide-based lipid scores may support laboratory-based cardiovascular risk stratification in clinically defined patient cohorts.
DOI Laboratory medicineClinical lipidomics in the era of the big data
Positions lipidomics as an emerging discipline within data-driven laboratory medicine and translational diagnostics.
DOI Inherited metabolic disordersRapid and efficient LC-MS/MS diagnosis of inherited metabolic disorders: a semi-automated workflow for analysis of organic acids, acylglycines, and acylcarnitines in urine
Robust, semi-automated LC-MS/MS workflows for clinical diagnostics of inherited metabolic disorders.
DOI Method developmentWide metabolite coverage LC-MS/MS assay for the diagnosis of inherited metabolic disorders in urine
A recent analytical study demonstrating broad metabolite coverage for diagnostic LC-MS/MS workflows in inherited metabolic disorders.
DOI Inflammation — goutAlterations in lipidome profiles distinguish early-onset hyperuricemia, gout, and the effect of urate-lowering treatment
Demonstrates how lipidomics can distinguish clinically relevant disease phenotypes and treatment effects in inflammatory and metabolic disease.
DOI Neurodegeneration — lipid metabolismChanges in lipid metabolism track with the progression of neurofibrillary pathology in tauopathies
A translational lipidomics study linking lipid metabolism to neurodegenerative disease progression and neurofibrillary pathology.
DOI Clinical metabolomics — laboratory medicineA global perspective on the status of clinical metabolomics in laboratory medicine — a survey by the IFCC metabolomics working group
Connects the group with the international discussion on how metabolomics is entering clinical laboratory medicine.
DOIThe complete record
List of all publications
Browse the full archive by year or search across titles, authors and journals. Every entry links to its DOI where available.
2026
6 papersA hybrid RNA-based reporter assay for robust quantification of cytidine deaminase activity
Derivatisation-free FIA-MS/MS assay for rapid simultaneous screening of atypical myopathy biomarkers and acylcarnitine profiles from dried blood spots
Long-Term Static Cultivation Alters Lipid Metabolism and Bioenergetic Capacity in A549 Cells
Aromatase Inhibitor Therapy Is Associated with Distinct Plasma Lipidomic Profiles in Postmenopausal Breast Cancer Patients
ABCA7 deficiency exacerbates glutamate excitotoxicity in Alzheimer's disease mice — a new pharmacological target for Glu-related neurotoxicity
2025
12 papersBest practices and tools in R and Python for statistical processing and visualization of lipidomics and metabolomics data
Ceramide-based risk score: a novel laboratory tool for cardiovascular risk stratification in hyperuricemia and gout
Cytidine and dCMP deaminases — current methods of activity analysis
Sex-dependent efficacy of sphingosine-1-phosphate receptor agonist FTY720 in mitigating Huntington's disease
Comparison of inflammatory biomarker levels in neurodegenerative proteinopathies: a case-control study
MInfer: bridging MetaboAnalyst and Jacobian analysis for metabolomic networks
The kinetics of uracil-N-glycosylase distribution inside replication foci
Horse with myopathy caused by consumption of box elder tree seedlings in the Czech Republic
Serum neopterin and mortality in lung cancer patients treated with immune checkpoint inhibitors
Multi-omics insights into human brown and white adipose tissue: identifying novel regulators of thermogenesis
Comprehensive metabolomic/lipidomic characterization of patients with mitochondrial ATP synthase, short-chain acyl-CoA dehydrogenase and combined variant deficiencies
Enhanced metabolomic predictions using concept drift analysis: identification and correction of confounding factors
2024
11 papersChanges in lipid metabolism track with the progression of neurofibrillary pathology in tauopathies
Patients with neurodegenerative proteinopathies exhibit altered tryptophan metabolism in the serum and cerebrospinal fluid
Parallel metabolomics and lipidomics of a PSMA/GCPII deficient mouse model reveal alteration of NAAG levels and brain lipid composition
A global perspective on the status of clinical metabolomics in laboratory medicine — a survey by the IFCC metabolomics working group
Assessing HCH isomer uptake in Alnus glutinosa: implications for phytoremediation and microbial response
Wide metabolite coverage LC-MS/MS assay for the diagnosis of inherited metabolic disorders in urine
Utilizing neurodegenerative markers for the diagnostic evaluation of amyotrophic lateral sclerosis
Long-chain polyunsaturated fatty acid-containing phosphatidylcholines predict survival rate in patients after heart failure
Adipokine levels of RBP4, resistin and nesfatin-1 in women diagnosed with gestational diabetes
Aminoacylase 1 deficiency: case report on three affected siblings
CERT score as a potential tool to predict cardiovascular risk in gout and hyperuricemia
2023
12 papersAlterations in lipidome profiles distinguish early-onset hyperuricemia, gout, and the effect of urate-lowering treatment
Clinical lipidomics in the era of the big data
From big data to better patient outcomes
Cerebrospinal fluid and blood serum biomarkers in neurodegenerative proteinopathies: a prospective, open, cross-correlation study
A new technique for the analysis of metabolic pathways of cytidine analogues and cytidine deaminase activities in cells
Rapid and efficient LC-MS/MS diagnosis of inherited metabolic disorders: a semi-automated workflow for analysis of organic acids, acylglycines, and acylcarnitines in urine
Principal balances of compositional data for regression and classification using partial least squares
Multivariate independent component analysis identifies patients in newborn screening equally to adjusted reference ranges
Determining thrombogenicity: using a modified thrombin generation assay to detect the level of thrombotic event risk in lupus anticoagulant-positive patients
Metabolomic profiling confirms the key role of oxidative stress in the pathophysiology of Diamond-Blackfan anemia
Selective pivot logratio coordinates for partial least squares discriminant analysis modelling with applications in metabolomics
Systematic comparison of advanced network analysis and visualization of lipidomics data
2022
11 papersLipidomic profiling of human serum enables detection of pancreatic cancer
Biomarkers of neurodegenerative diseases: biology, taxonomy, clinical relevance, and current research status
Specific gut bacterial and fungal microbiota pattern in the first half of pregnancy is linked to the development of gestational diabetes mellitus in the cohort including obese women
Lipidomic and metabolomic analysis reveals changes in biochemical pathways for non-small cell lung cancer tissues
Metabolomic, lipidomic and proteomic characterisation of lipopolysaccharide-induced inflammation mouse model
Altered plasma, urine, and tissue profiles of sulfatides and sphingomyelins in patients with renal cell carcinoma
Dynamics of acylcarnitines, hypoglycin A, methylenecyclopropylglycine and their metabolites in a Kladruber stallion with atypical myopathy
Determination of thrombogenicity levels of various antiphospholipid antibodies by a modified thrombin generation assay in patients with suspected antiphospholipid syndrome
Combined targeted and untargeted profiling of HeLa cells deficient in purine de novo synthesis
Targeted plasma lipidomics distinguishes patients with gout and hyperuricemia from controls
Metabolomic analysis of HeLa cells deficient in purine de novo synthesis
2021
7 papersImpact of newborn screening and early dietary management on clinical outcome of patients with long chain 3-hydroxyacyl-CoA dehydrogenase deficiency and medium chain acyl-CoA dehydrogenase deficiency — a retrospective nationwide study
Metabolomic and lipidomic changes triggered by lipopolysaccharide-induced systemic inflammation in transgenic APdE9 mice
GM3 ganglioside linked to neurofibrillary pathology in a transgenic rat model for tauopathy
SLIDE — novel approach to apocrine sweat sampling for lipid profiling in healthy individuals
Novel LC-MS tools for diagnosing inborn errors of metabolism
Evaluation of the determination of dabigatran, rivaroxaban, and apixaban in lupus anticoagulant-positive patients
Plasma short-chain fatty acids and their derivatives in women with gestational diabetes mellitus
2020
4 papersCROP: correlation-based reduction of feature multiplicities in untargeted metabolomic data
Cellwise outlier detection and biomarker identification in metabolomics based on pairwise log ratios
Bayesian multiple hypotheses testing in compositional analysis of untargeted metabolomic data
Global DNA methylation in rats' liver is not affected by hypercholesterolemic diet
2019
5 papersEvaluation of the DOAC-Stop procedure by LC-MS/MS assays for determining the residual activity of dabigatran, rivaroxaban, and apixaban
Epidemiology of rare diseases detected by newborn screening in the Czech Republic
Impact of sample dimensionality on orthogonality metrics in comprehensive two-dimensional separations
Urease-immobilized magnetic microparticles in urine sample preparation for metabolomic analysis by gas chromatography-mass spectrometry
White adipose tissue response of obese mice to ambient oxygen restriction at thermoneutrality: response markers identified, but no WAT inflammation
2018
7 papersMass spectrometric analysis of purine de novo biosynthesis intermediates
Structural elucidation of novel biomarkers of known metabolic disorders based on multistage fragmentation mass spectra
Human white adipose tissue metabolome: current perspective
Newborn foal with atypical myopathy
Multianalyte determination of NOACs using LC-MS/MS and comparison with functional coagulation assays
Radio-sensitizing effects of VE-821 and beyond: distinct phosphoproteomic and metabolomic changes after ATR inhibition in irradiated MOLT-4 cells
Toll-like receptor 7/8 ligand, S28463, suppresses Ascaris suum-induced allergic asthma in nonhuman primates
2017
4 papersMetabolic response of visceral white adipose tissue of obese mice exposed for 5 days to human room temperature compared to mouse thermoneutrality
Metabolic status of CSF distinguishes rats with tauopathy from controls
Novel sulphur-containing imatinib metabolites found by untargeted LC-HRMS analysis
Sample-independent approach to normalize two-dimensional data for orthogonality evaluation using whole separation space scaling
2016
9 papersMetabolite profiling of the plasma and leukocytes of chronic myeloid leukemia patients
Influence of mass resolving power in orbital ion-trap mass spectrometry-based metabolomics
Citrulline as a biomarker of gastrointestinal toxicity in patients with rectal carcinoma treated with chemoradiation
Dr Jekyll and Mr Hyde: a strange case of 5-ethynyl-2′-deoxyuridine and 5-ethynyl-2′-deoxycytidine
A lower dosage of imatinib is sufficient to maintain undetectable disease in patients with chronic myeloid leukemia with long-term low-grade toxicity of the treatment
Equine atypical myopathy: a metabolic study
Normalization techniques for PARAFAC modeling of urine metabolomic data
Ultrafast online SPE-MS/MS method for quantification of 3 tyrosine kinase inhibitors in human plasma
Amlodipine plasma levels and side effects in patients with arterial hypertension
2015
4 papersOxidized phosphatidylcholines suggest oxidative stress in patients with medium-chain acyl-CoA dehydrogenase deficiency
A fatal combination: a thymidylate synthase inhibitor with DNA damaging activity
Untargeted metabolomic analysis of urine samples in the diagnosis of some inherited metabolic disorders
Detailed study of imatinib metabolization using high-resolution mass spectrometry
2014
2 papersFenretinide prevents inflammation and airway hyperresponsiveness in a mouse model of allergic asthma
Influence of mass spectrometry resolution on metabolite coverage in plasma
2013
2 papersA significant proportion of patients with chronic myeloid leukemia and suboptimal response according to European LeukemiaNet criteria have excellent prognosis without treatment change
Metabolomic techniques in biomedicine
2012
5 papersTargeted metabolomic analysis of plasma samples for the diagnosis of inherited metabolic disorders
Isotope dilution direct injection mass spectrometry method for determination of four tyrosine kinase inhibitors in human plasma
Imatinib trough plasma levels do not correlate with the response to therapy in patients with chronic myeloid leukemia in routine clinical setting
Analysis of cytokinin nucleotides by capillary zone electrophoresis with diode array and mass spectrometric detection in a recombinant enzyme in vitro reaction
High lapatinib plasma levels in breast cancer patients: risk or benefit?
2011
1 paperAnalysis of nucleotides in dry blood spots
2010
7 papersFlow injection analysis vs. ultra high performance liquid chromatography coupled with tandem mass spectrometry for determination of imatinib in human plasma
Lapatinib in breast cancer — the predictive significance of HER1 (EGFR), HER2, PTEN and PIK3CA genes and lapatinib plasma level assessment
Imatinib dose escalation in two patients with chronic myeloid leukemia, with low trough imatinib plasma levels measured at various intervals from the beginning of therapy and with suboptimal treatment response, leads to the achievement of higher plasma levels and major molecular response
Determination of tyrosine kinase inhibitors in human plasma by UHPLC-MS/MS
Oligodendroglia from ADSL-deficient patient produce SAICAribotide and SAMP
Measurement of trough imatinib plasma levels in patients with CML does not significantly correlate with treatment response but may be successfully used in selected patients for dosage adjustment
Nucleotide analysis in blood spots
2009
1 paperCapillary electrophoresis determination of thiopurine methyl transferase activity in erythrocytes
2008
3 papersImatinib plasma levels correlate with molecular response in CML patients
ITPase activity in dry blood spots is comparable with that in fresh erythrocytes
Determination of imatinib in plasma by high performance capillary electrophoresis
2007
4 papersCapillary electrophoretic method for nucleotide analysis in cells: application on inherited metabolic disorders
Analysis of aminoimidazole ribosides by capillary electrophoresis: diagnosing defects in second part of purine biosynthetic pathway
Determination of ITPase activity by capillary electrophoresis
Spectral and electromigration characteristics of imidazole riboside
2006
5 papersDiagnosing AICA-ribosiduria by capillary electrophoresis
Analysis of intracellular nucleotides by capillary electrophoresis — mass spectrometry
Synthesis and mass spectrometric fragmentation characteristics of imidazole ribosides — analogs of intermediates of purine de novo synthetic pathway
Analysis of aminoimidazole ribosides by capillary electrophoresis: diagnosing inborn errors of second part of purine de novo synthesis
Screening for defects in purine de novo biosynthesis by TLC
2005
1 paperThe involvement of cytokinin oxidase/dehydrogenase and zeatin reductase in regulation of cytokinin levels in pea (Pisum sativum L.) leaves
2004
1 paperAnalytical derivatization — a tool for determination of orotic acid
2003
1 paperHigh-throughput capillary electrophoretic method for determination of total aminothiols in plasma and urine
2002
2 papersCapillary electrophoresis for detection of inherited disorders of purine and pyrimidine metabolism: a selective approach
Screening method for inherited disorders of purine and pyrimidine metabolism by capillary electrophoresis with reversed electroosmotic flow
2001
1 paperFast analysis of antibacterial isothiazolones by capillary electrophoresis
2000
1 paperMass spectrometry for analysis of purine and pyrimidine compounds
1999
2 papersCapillary electrophoresis for detection of inherited disorders of purine and pyrimidine metabolism
New techniques in diagnostics by capillary electrophoresis of hereditary disorders of purine and pyrimidine metabolism
1998
1 paperStudy of cytokinin separation using capillary electrophoresis with cyclodextrin additives
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